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Enterobacteria phage T4. Escherichia virus T4 is a species of bacteriophages that infect Escherichia coli bacteria. It is a double-stranded DNA virus in the subfamily Tevenvirinae of the family Straboviridae. T4 is capable of undergoing only a lytic life cycle and not the lysogenic life cycle.
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. T m depends on the length of the DNA molecule and its specific ...
T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified [1] as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. [2] Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. [3]
T4 rII system. The T4 rII system is an experimental system developed in the 1950s by Seymour Benzer for studying the substructure of the gene. The experimental system is based on genetic crosses of different mutant strains of bacteriophage T4, a virus that infects the bacteria Escherichia coli .
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA).
From 2002 to 2010, a team at the Hungarian Academy of Science created a strain of Escherichia coli called MDS42, which is now sold by Scarab Genomics of Madison, WI under the name of "Clean Genome E. coli", [123] where 15% of the genome of the parental strain (E. coli K-12 MG1655) were removed to aid in molecular biology efficiency, removing IS ...
Temperature determines the statistical occupation of the microstates of the ensemble. The microscopic definition of temperature is only meaningful in the thermodynamic limit, meaning for large ensembles of states or particles, to fulfill the requirements of the statistical model. Kinetic energy is also considered as a component of thermal energy.
In biology a thermal optimum describes the ideal boundaries for biological processes such as growth and development, and is usually characteristic of a species or population. Most biological processes are dependent upon enzymatic activity that can be impacted by the organism's body temperature, which in term is a function of the organism's ...