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The 3-D structure is held together with hydrogen bonds and can therefore be disrupted by heating back to a liquid state. The melting temperature is different from the gelling temperature, depending on the sources, agarose gel has a gelling temperature of 35–42 °C and a melting temperature of 85–95 °C.
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...
NPU terminology. NPU terminology (NPU: Nomenclature for Properties and Units) is a patient centered clinical laboratory terminology for use in the clinical laboratory sciences. Its function is to enable results of clinical laboratory examinations to be used safely across technology, time and geography. To achieve this, the NPU terminology supplies:
As heat blankets much of the US, hospitals in many states are already seeing extremely high rates of heat-related emergencies, according to data compiled by the U.S. Centers for Disease Control ...
Ten alleged members of a Minneapolis gang accused of “terrorizing” a city neighborhood have been charged with a range of federal crimes including possession of a machine gun and drug ...
Commissioners in Nevada’s second most populous county on Tuesday refused to certify the results of two local recounts from last month’s primary, a rare move that has potential implications for ...
In touchdown PCR, the annealing temperature is gradually decreased in later cycles. The annealing temperature in the early cycles is usually 3–5 °C above the standard T m of the primers used, while in the later cycles it is a similar amount below the T m. The initial higher annealing temperature leads to greater specificity for primer ...
Operating temperature. An operating temperature is the allowable temperature range of the local ambient environment at which an electrical or mechanical device operates. The device will operate effectively within a specified temperature range which varies based on the device function and application context, and ranges from the minimum ...