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Sudan stain. Sudan stains and Sudan dyes are synthetic organic compounds that are used as dyes for various plastics (plastic colorants) and are also used to stain sudanophilic biological samples, usually lipids.
Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. These Sudan groups of dyes are defined as lysochrome dyes which are soluble in fats and lipids and also lipid solvents.
Sudan Black B (SBB) is a fat soluble dye which has very high affinity for neutral fats and lipids. SBB staining is useful for for the differentiation of Acute myeloid leukemia (AML) from Acute lymphoid leukemia (ALL).
The structure within the phloem of higher plants that is responsible for transporting organic material (sucrose, raffinose, amino acids, etc.) from the photosynthetic tissues (e.g. leaves) to other parts of the plant. Made up of a column of cells (sieve elements) connected by sieve plates.
The staining with a saturated solution of Sudan black B in 70% alcohol indicates the presence of unsaturated fatty acids, as does its blackening with aqueous solutions of osmium tetroxide and silver nitrate and ready staining with iodine (Bondareff, 1964).
Principle. As SBB is a fat-soluble dye, it discolours lipids, including sterols, neutral fats, and phospholipids. These are present in the azurophilic, secondary, and lysosomal granules of myelocytic and monocytic cells, respectively. As a result of its greater solubility in lipids than in solvent, the dye leaves the solvent during staining.
Sudan Black B is a fat-soluble diazo dye which stains neutral triglycerides and lipids. From: Bancroft's Theory and Practice of Histological Techniques (Eighth Edition), 2019. About this page. Add to Mendeley. Chapters and Articles. You might find these chapters and articles relevant to this topic. Erythrocyte and leucocyte cytochemistry.
As its name suggests, Sudan Black B stains lipofuscins black. It is commonly used to stain lipids and fats, hence the fact it stains lipofuscins is important. It can also stain red blood cells black as well. To conduct a Sudan Black B stain, the following steps are involved: The sample is hydrated using alcohol
Quantitation of stool fat over 48 or 72 h is the ideal way to assess steatorrhea, although a quantitative measurement (Sudan stain) is a good screening test. However, moderately increased fecal fat excretions (^ 14 g/day) may also result from altered small-bowel motor or secretory function.
Sudan IV (C 24 H 20 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520 (357) nm.